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transplex ® complete whole transcriptome amplification kit  (Millipore)


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    Structured Review

    Millipore transplex ® complete whole transcriptome amplification kit
    Transplex ® Complete Whole Transcriptome Amplification Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transplex%C2%AE+complete+whole+transcriptome+amplification+kit/wta2+kit/pmc11356395-63-6-13
    Average 90 stars, based on 1 article reviews
    transplex ® complete whole transcriptome amplification kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Generated:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Amplification:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Whole Genome Amplification:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Expressing:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Isolation:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Standard Deviation:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Transfection:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Binding Assay:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Activity Assay:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Western Blot:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.

    Staining:

    Article Title: Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors
    Article Snippet: RNA was isolated with a RNA clean XP Kit (Agencourt Bioscience). cDNA synthesis and amplification was performed by a TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2–50RXN, Sigma-Aldrich).

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantiTectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: Autophagy-mediated NCOR1 degradation is required for brown fat maturation and thermogenesis.
    Article Snippet: Brown adipose tissue (BAT) thermogenesis affects energy balance, and thereby it has the potential to induce weight loss and to prevent obesity.. Here, we document a macroautophagic/autophagicdependent mechanism of peroxisome proliferator-activated receptor gamma (PPARG) activity regulation that induces brown adipose differentiation and thermogenesis and that is mediated by TP53INP2.. Disruption of TP53INP2-dependent autophagy reduced brown adipogenesis in cultured cells.

    Article Title: Regulation of tumor angiogenesis and mesenchymal–endothelial transition by p38α through TGF-β and JNK signaling
    Article Snippet: Total RNA was extracted from MSCs using PureLink RNA kit (Ambion #12183018 A) and 25 ng were amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2, Sigma Aldrich).

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs.
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Method for identifying novel minor histocompatibility antigens
    Article Snippet: Kits suitable for transcriptome library construction are commercially available, for example from Life Technologies (Ambion® RNA-Seq Library Construction Kit), Applied Biosystems (AB Library BuilderTM Whole Transcriptome Core Kit), Qiagen (QuantilectTM Whole Transcriptome Kit) and Sigma-Aldrich (TransPlex® Complete Whole Transcriptome Amplification Kit) In an embodiment, genomic libraries are generated/constructed from the genomic DNA obtained from the sample.

    Article Title: A new highly divergent copiparvovirus in sheep.
    Article Snippet: The subfamily Parvovirinae within the family Parvoviridae consists of viruses that can infect a wide range of vertebrate hosts and cause effects ranging from severe disease to asymptomatic infection.. In the present study, high-throughput sequencing (HTS) was utilized to analyze samples obtained from an abortion outbreak in a sheep flock to identify a putative viral etiology.. A highly divergent nearly complete parvovirid genome sequence, approximately 4.9 kb in length, was determined.

    Article Title: Virome of crab-eating ( Cerdocyon thous ) and pampas foxes ( Lycalopex gymnocercus ) from southern Brazil and Uruguay
    Article Snippet: Furthermore, the viral RNA was reverse-transcribed using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich) following the manufacturer's recommendations.



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    Whole <t>transcriptome</t> analysis was performed on the left ventricle (LV), right ventricle (RV), left atrium (LA) and the right atrium (RA) of rhesus monkey (Macaca mulatta) fetuses that were exposed maternally to a 400 µg/kg body weight, Bisphenol A (BPA), relative to matched control fetuses, either during, (A) early gestation, EG (days; 50–100±2) or, (B) late gestation, LG (days 100±2–term). Genes that changed by greater than ±1 log 2 fold change (1 LFC = 2 fold change) at an unadjusted p≤0.01 were considered significant and differentially expressed. Bars represent the number of upregulated (positive y-axis) or downregulated (negative y-axis) genes in each of the tissues.
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    Whole transcriptome analysis was performed on the left ventricle (LV), right ventricle (RV), left atrium (LA) and the right atrium (RA) of rhesus monkey (Macaca mulatta) fetuses that were exposed maternally to a 400 µg/kg body weight, Bisphenol A (BPA), relative to matched control fetuses, either during, (A) early gestation, EG (days; 50–100±2) or, (B) late gestation, LG (days 100±2–term). Genes that changed by greater than ±1 log 2 fold change (1 LFC = 2 fold change) at an unadjusted p≤0.01 were considered significant and differentially expressed. Bars represent the number of upregulated (positive y-axis) or downregulated (negative y-axis) genes in each of the tissues.

    Journal: PLoS ONE

    Article Title: Maternal Bisphenol A Exposure Impacts the Fetal Heart Transcriptome

    doi: 10.1371/journal.pone.0089096

    Figure Lengend Snippet: Whole transcriptome analysis was performed on the left ventricle (LV), right ventricle (RV), left atrium (LA) and the right atrium (RA) of rhesus monkey (Macaca mulatta) fetuses that were exposed maternally to a 400 µg/kg body weight, Bisphenol A (BPA), relative to matched control fetuses, either during, (A) early gestation, EG (days; 50–100±2) or, (B) late gestation, LG (days 100±2–term). Genes that changed by greater than ±1 log 2 fold change (1 LFC = 2 fold change) at an unadjusted p≤0.01 were considered significant and differentially expressed. Bars represent the number of upregulated (positive y-axis) or downregulated (negative y-axis) genes in each of the tissues.

    Article Snippet: Due to the limitations in the quantity of tissue available from the LA and RA, cDNA preparations from both these tissue were prepared by employing a PCR based amplification approach available through TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2) (Sigma, St. Louis, MO, USA).

    Techniques: